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Literature summary extracted from

  • Patil, R.V.; Dekker, E.E.
    Cloning, nucleotide sequence, overexpression, and inactivation of the Escherichia coli 2-keto-4-hydroxyglutarate aldolase gene (1992), J. Bacteriol., 174, 102-107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.42
-
 Escherichia coli
4.1.3.42 overexpressed in pKK223.3 expression vector Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.42 additional information gene inactivation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.42 2.6
-
 (4S)-4-hydroxy-2-oxoglutarate pH 8.4, 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.42 additional information no requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.42 22000
-
 3 * 22284, calculated, 3 * 22000, SDS-PAGE Escherichia coli
4.1.3.42 22284
-
 3 * 22284, calculated, 3 * 22000, SDS-PAGE Escherichia coli
4.1.3.42 62500
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.42 Escherichia coli
-
-
-
4.1.3.42 Escherichia coli
-
 overexpressed after cloning into pKK223.3 expression vector
-
4.1.3.42 Escherichia coli P0A955
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.42 additional information
-
 80fold increase in specific activity after overexpression in pKK223.3 Escherichia coli
4.1.3.42 45
-
 pH 8.4, 25°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.42 (4S)-4-hydroxy-2-oxoglutarate
-
 Escherichia coli pyruvate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.42 trimer 3 * 22284, calculated, 3 * 22000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.1.3.42 2-keto-4-hydroxybutyrate aldolase ambiguous Escherichia coli
4.1.3.42 2-keto-4-hydroxyglutarate aldolase ambiguous Escherichia coli
4.1.3.42 2-keto-4-hydroxyglutaric aldolase ambiguous Escherichia coli
4.1.3.42 2-oxo-4-hydroxyglutarate aldolase ambiguous Escherichia coli
4.1.3.42 2-oxo-4-hydroxyglutaric aldolase ambiguous Escherichia coli
4.1.3.42 4-hydroxy-2-ketoglutarate aldolase ambiguous Escherichia coli
4.1.3.42 4-hydroxy-2-ketoglutaric aldolase ambiguous Escherichia coli
4.1.3.42 4-hydroxy-2-oxoglutarate glyoxylate-lyase ambiguous Escherichia coli
4.1.3.42 eda
-
 Escherichia coli
4.1.3.42 HGA
-
 Escherichia coli
4.1.3.42 hydroxyketoglutarate aldolase ambiguous Escherichia coli
4.1.3.42 Hydroxyketoglutaric aldolase ambiguous Escherichia coli
4.1.3.42 KHG/KDPG aldolase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.42 8.4
-
-
 Escherichia coli